Raw data for the article "Glucose restriction induces degeneration of neurons with mitochondrial DNA depletion by altering ER-mitochondria calcium transfer"

Total RNA was extracted with TRIzol. Libraries were prepared using an Illumina TruSeq RNA Sample Prep kit (Illumina, 20020594) following the manufacturer’s instruction. The RNA-seq experiment was performed at Annoroad Gene Technology Co. Ltd. (Beijing, China). The raw reads of RNA-seq were trimmed from 3′ to 5′ ends for each read, and the reads shorter than 50 bp were discarded. The reads that passed the quality control were mapped to mouse genome (mm10) with STAR (version 2.7). Stringtie (version 2.1.4) were then used to estimate transcript abundance in fragments per kilobase per million (FPKM). Genes were annotated as differentially expressed using DESeq2 (version 1.44). The GO analysis and KEGG pathway analysis were performed using R packages.