MEF2C knock-down in Human Skeletal Myoblasts [HSkM]

The main goal of this work was to determine the connection between MEF2C expression and its role in miRNA biogenesis in the differentiation of human skeletal muscle cells.In summary, our results demonstrated a significant role for MEF2C myogenic factor in sculpting the microtranscriptome during the differentiation of skeletal muscle cells. Overall design: miRNAs libraries from HSkM cell treated with control siRNA (n=2) and two separate siRNA against MEF2C: siMEF2C_1 (n=2) and siMEF2C_2 (n=2) were generated by deep sequencing, using the NovaSeq 6000