Genome-wide analysis of mechano-responsive gene expression by tenocytes in fascicles subjected to cyclic tensile strain. Rattus norvegicus

Analysis of mechano-regulation of tenocyte metabolism at gene expression level. The hypothesis tested in the present study was that cyclic tensile strain influence the balance of anabolism/catabolism of tenocytes. Results provide important information of the response of tenocyte to cyclic tensile strain, such as specific mechano-responsive genes, up- or down-regulated specific anabolic/catabolic cellular functions. Keywords: gene expression array-based, stress response, functional analysis Overall design: Total RNA obtained from isolated tendon fascicles subjected to 1 or 24 hours in vitro cyclic tensile strain was analysed in triplicate by comparison with unstrained control fascicles. A total of 12 RNA samples were analysed (3 samples each from strained and unstrained group at each time point).