TIRF Microscopy Image Sequences of FcεRI-Centric Synapse Formation in RBL-2H3 Cells Dataset

1. Introduction Total internal reflection fluorescence (TIRF) microscopy was used to image the IgE-FcεRI receptor signaling complex in rat basophilic leukemia (RBL-2H3) cells coming into contact with a supported lipid bilayer, modeling an immunological model synapse. When mast cells contact a monovalent antigen-bearing fluid lipid bilayer, IgE-loaded FcεRI receptors aggregate (forming receptor clusters) at contact points and trigger degranulation and the release of immune activators. After aggregation, micron-sized cluster move by apparently performing diffusion or random walk motion [1] to eventually coalesce to form a big FcεRI-centric receptor patch, called the mast cell synapse [2]. 2. Data Info This data sets contains microscope image sequences of this synapse formation. The data are provided as Image Cytometry Standard (.ics) files with corresponding .ids files. These files can be readily opened with ImageJ (also known as Fiji), see: https://imagej.net/Fiji. Image exposure was 50 ms or 100 ms and pixel size was 167 nm. Images were taken every second. Image exposure is indicated in the folder name. A total of 8 time series or movies of RBL-2H3 cells forming this big FcεRI-centric receptor patch are included in this data set.