Influence of Coaggregated E. faecalis and F. nucleatum on the Transcriptome of Macrophage

Refractory apical periodontitis (RAP), a persistent infection after root canal treatment, still has no effective treatment. Enterococcus faecalis (E. faecalis) and Fusobacterium nucleatum (F. nucleatum) are frequently detected in the lesion. We previously found that coaggregation altered gene expression of E. faecalis and F. nucleatum and promotes immune evasion by suppressing pro-inflammatory cytokine secretion of macrophage , while sustaining low-grade inflammation. In this study, we further investigated the synergistic effect of coaggregated E. faecalis and F. nucleatum on modulating macrophage transcriptional profiling, in order to offer novel insights into therapeutic targets for RAP.