Proteomics of 24 h TCR activated wild-type and Pim1/Pim2 double knockout CD4 and CD8 T cells

T cell antigen-receptor (TCR) and cytokine receptor engagement trigger large changes in Serine/Threonine kinase signalling networks to drive T cell activation and differentiation. The role of only few kinase signalling pathways have been studied in detail, and in this context, Pim kinases are an interesting, yet understudied, family of Serine/Threonine kinases, with reported roles in key processes including survival, proliferation, metabolism across a range of cell types. T lymphocytes predominantly express PIM1 and PIM2, which are rapidly induced by TCR, costimulation and cytokine signalling. Using high-resolution mass spectrometry we systematically examine the impact of Pim1/Pim2 double deficiency downstream of initial TCR engagement. We find that Pim kinases are dispensable for TCR-driven T cell activation and have little impact on the total proteome in these cells.