Genome-wide map of TRIM33 binding sites in primary dendritic cells

The homeostatic control of dendritic cells (DCs) is critical for appropriate immune responses, yet the regulatory mechanisms are not fully elucidated. Genome-wide mapping of TRIM33 binding sites in primary DCs revealed a role of this molecule in DC transcription regulation, and therefore homeostasis. Overall design: Chromatin immunoprecipitation-sequencing (ChIP-seq) was performed to determine genome-wide binding sites of TRIM33 in plasmacytoid dendritic cells (pDCs) as well as type 1 and 2 conventional dendritic cells (cDC1s, cDC2s). Primary DCs were sorted from wildtype C57BL/6 mice, and 2 biological repeats were prepared for each subset and subjected to ChIP assays with anti-TRIM33 antibody. Immunoprecipitated DNA was sequenced on the Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads.