A recurrent neural circuit in Drosophila temporally sharpens visual inputs

A critical goal of vision is to detect changes in light intensity, even when these changes are blurred by the spatial resolution of the eye and the motion of the animal. Here we describe a recurrent neural circuit in Drosophila that compensates for blur and thereby selectively enhances the perceived contrast of moving edges. Using in vivo, two-photon voltage imaging, we measured the temporal response properties of L1 and L2, two cell types that receive direct synaptic input from photoreceptors. These neurons have biphasic responses to brief flashes of light, a hallmark of cells that encode changes in stimulus intensity. However, the second phase was often much larger in area than the first, creating an unusual temporal filter. Genetic dissection revealed that recurrent neural circuitry strongly shapes the second phase of the response, informing the structure of a dynamical model. By applying this model to moving natural images, we demonstrate that rather than veridically representing st..., This dataset was collected using two-photon microscopes (Leica and Bruker) and Lightcrafter projectors controlled with custom MATLAB data (see \"stimulus\" folder on Github). These imaging and stimulus data were processed with custom MATLAB data to draw ROIs, extract deltaF/F traces, and generate stimulus-aligned averages (see \"imaging-analysis\" folder on Github)., , # A recurrent neural circuit in Drosophila temporally sharpens visual inputs

[https://doi.org/10.5061/dryad.ngf1vhj4c](https://doi.org/10.5061/dryad.ngf1vhj4c)

## Description of the data and file structure

This dataset contains a metadata spreadsheet, MATLAB files containing analyzed data, and zip files containing raw data.

### Metadata spreadsheet

The metadata for all experiments are saved in one spreadsheet (L1L2_Metadata.xlsx), with tabs organized by experiment/figure, except for the first tab (\"List of flies\") which lists the dates/flies corresponding to each experiment, and the second tab (\"All Metadata\") which contains metadata for all experiments without extra whitespace and can be saved separately as a tab-delimited note file for use in further analysis if needed.

The rows and columns (in all sheets except for the list of flies) are organized as follows, with the header names in bold (there are some differences depending on if recordings were acquired with a Leica or Bruke...