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Reconstitution of Pluripotency from Mouse Fibroblast through Sall4 Overexpression

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We report that SALL4 alone, under an optimized reprogramming medium iCD4, is capable of reprogramming mouse fibroblasts into iPSCs. To identify SALL4-direct-interacting proteins, we performed immunoprecipitation followed by mass spectrometry (IP-MS) using MEFs overexpressing WT-SALL4 or SALL4 mutants (ΔZFC1, ΔZFC2, ΔZFC3, and ΔN12) at day 1.

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2024-11-05
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