Disruption of RNAPII transcription elongation links Oncogenic splicing factor mutations to replciation stress and targetable alterations in chromatin landscape [DRIP-seq]

To investigate the distribution of R-loop formation, we peformed R-loop mapping analysis using S9.6 antibody-based DRIP-seq in K562 cells (wild type and SF3B1K700E mutant) at 4 days after mutant allele expression We then performed metaplot and peak-based analysis using data obtained from DRIP-seq R-loop analysis for K562 (wild type vs SF3B1K700E mutant) cells