Gene expression analysis of Kasumi9 cells cultured with or without BCAA. Gene expression analysis of Kasumi9 cells cultured with or without BCAA

The branched-chain amino acid (BCAA) metabolism plays pleiotropic roles in homeostasis. Here we show that human acute leukemia-initiating cells (LICs), but not normal hematopoietic stem cells, are heavily addicted to the BCAA metabolism, irrespective of myeloid or lymphoid types. To clarify how BCAA metabolism affect the gene expression of human acute leukemia cells, we examined the gene expression alteration in human acute leukemia cell lines in control and BCAA-resrticted culture conditions. Overall design: To clarify the global gene expression alteration in ALL cells after BCAA metabolism inhibition, Kasumi9 cells were cultured with or without BCAA. After 8-10 days of culture, FACS-purified Kasumi9 cells were subjected to transcriptome analysis.