qPCR data for archaeal amoA ecotypes; samples collected on METZYME cruise.
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<p>Abundance of ammonia monooxygenase subunit A (<em>amoA</em>) genes determined using TaqMan quantitative polymerase chain reaction (qPCR). Archaeal <em>amoA</em> genes were quantified using quantitative PCR using the assay described in Mosier and Francis 2011. Complete methods are described in Santoro et al., submitted.</p>
<p><strong>Related references: </strong><br />
Mosier, A. C., and C. A. Francis. 2011. Determining the distribution of marine and coastal ammonia-oxidizing archaea and bacteria using a quantitative approach. Methods Enzymol. 486<strong>: </strong>205-221. doi:<a href=\"http://dx.doi.org/10.1016/B978-0-12-381294-0.00009-2\" target=\"_blank\">10.1016/B978-0-12-381294-0.00009-2</a></p>
<p>Santoro, A.E., Saito, M.A., Goepfert, T.J., Lamborg, C.H., Dupont, C.L., G.R. DiTullio. Thaumarchaeal ecotype distributions across the equatorial Pacific and their potential roles in nitrification and flux attenuation. Submitted to <em>Limnology & Oceanography, April 2016</em>.</p>
创建时间:
2021-12-05



