Ruffles induction upon ASFV entry in Vero cells.

A) Field Emission SEM of mock-infected and infected cells. Cells were serum starved for 24 h and synchronously infected for 10, 60 and 90 min (MOI 50). A magnification of the cell surface detail (boxes) is shown in the lower right panels. Arrowheads indicate ruffles and arrows indicate bubble-like membrane perturbations. B) TEM of purified viral particle (arrows) localized into ruffles (arrowheads) in the cells after binding for 90 min at 4°C (MOI 3000). C) Ruffle induction upon ASFV binding to Vero cells. After being serum starved for 24 h, virus binding was allowed for 90 min at 4°C (MOI 100), and infected cells were recorded during 30 min after warming at 37°C with a 20× objective. Time stamps indicate min: sec.