RNA-sequencing of wild-type and ArhGEF1-deficient splenic cDC2s. RNA-sequencing of wild-type and ArhGEF1-deficient splenic cDC2s

We analyzed the transcriptome differences of wild-type and ArhGEF1-deficient (Arhgef1-/-) type-2 conventional dendritic cells (cDC2s) in spleen. Wild-type or ArhGEF1-deficient (Arhgef1-/-) bone marrow cells were transferred into lethal irradiated CD45.1 mice. 8 weeks later, splenic cDC2s were purified and prepared for RNA-seq analysis. The gene expression profiles of CD97-, Gα13- and ArhGEF1-deficient cDC2s were highly similar, providing evidence that these molecules function in the same pathway. Overall design: Transcriptome analysis of WT and Arhgef1-/- splenic cDC2s