The effect of Filamin A knockdown on mRNA expression profile in human SaOS2 cells

Filamin A (FLNA) is involved in a variety of cellular processes. However, how FLNA regulates genomewide mRNA expression in cancer cells remains unclear. Using biological and mRNA-seq techniques, we generated the SaOS2 cell lines stably expressing FLNA shRNA or control shRNA and extracted the total RNA from these cell lines. The resulting RNA was subjected to mRNA sequencing. The data from the mRNA-seq can be used for the analysis of differential expression genes and the pathways that are modulated by FLNA. In other words,the goal of this project is to understand how many genes or biological pathways can be regulated by FLNA in SaOS2 cells. The finding from this project would provide a novel insight into the role of FLNA in genomewide mRNA expression in cancer cells.