Identification, visualization and clonal analysis of intestinal stem cells in fish

Movie 1: The rostral part of the medaka intestinal tract. A 3D view of X-ray data starting from the buccal cavity and going through the mid-gut reveals distinct topographical regions along the rostro-­‐caudal axis. Movie 2: The caudal part of the medaka intestinal tract. A 3D view of X-ray data from the hind-gut indicating the narrower passage in the caudal part of the intestine with smaller folds. Movie 3: The GaudíRSGubiquitin:ERT2Cre (10dpi) MuVi-Spim 3D visualization of a gut segment at 10days after induction shows already labeling of strings of clonally related groups representing the stem cells and their descendants (green). Movie 4: The GaudíRSGubiquitin:ERT2Cre (30dpi) MuVi-Spim 3D visualization of a gut segment at 30 days after induction showing intestinal folds that are either broadly labelled by H2B-­‐ EGFP (green), or not at all (grey) indicating longitudinal expansion of the clones. Movie 5: The GaudíRSGubiquitin:ERT2Cre (150dpi) MuVi-Spim 3D visualization of a gut segment at 150 days after induction. Two large clones (in green) established by symmetric division and neutral drift. Movie 6: The GaudíLxBBW hsp70:nlsCRE (90dpi) MuVi-Spim 3D visualization of a gut segment 90 days after induction shows that stochastically triggered clonal multi-color labeling results in adjacent clones of different sizes discernible by different fluorescent proteins demonstrated in this movie by yellow, green, blue and purple color.