AGO10-dependent gene expression under cytokinin treatment and control conditions

To investigate the role of AGO10 in the buffering of cytokinin responses we grew the ago10-1 mutant and the Col-0 wildtype on plates for six daysbefore treting with mock solution or cytokinin for 2 hours. Then root tips excised and RNA was extracted using a commercial kit. Overall design: Comparative gene expression analysis of Col-0 and ago10-1 root tips grown on half-strength MS medium mock treated or treated with the synthetic cytokinin 6-Benzyladenine