Data from: The brassinosteroid receptor gene BRI1 safeguards cell-autonomous brassinosteroid signaling across tissues

Brassinosteroid signaling is essential for plant growth as exemplified by
the dwarf phenotype of loss-of-function mutants in BRASSINOSTEROID
INSENSITIVE 1 (BRI1), a ubiquitously expressed Arabidopsis brassinosteroid
receptor gene. Complementation of brassinosteroid-blind receptor mutants
by BRI1 expression with various tissue-specific promoters implied that
local brassinosteroid signaling may instruct growth non-cell-autonomously.
Here we performed such rescues with a panel of receptor variants and
promoters, in combination with tissue-specific transgene knockouts. Our
experiments demonstrate that brassinosteroid receptor expression in
several tissues is necessary but not sufficient for rescue. Moreover,
complementation with tissue-specific promoters requires the genuine BRI1
gene body sequence, which confers ubiquitous expression of trace receptor
amounts that are sufficient to promote brassinosteroid-dependent root
growth. Our data, therefore, argue for a largely cell-autonomous action of
brassinosteroid receptors.