Single-cell sequencing from the same pool of cells (identical barcodes) to generate short and long reads.

To understand gene regulation and function requires a genome-wide method capable of capturing both gene expression levels and isoform diversity at the single-cell level. An exponentially growing fixed human cell line was used for droplet-based single-cell mRNA transcriptome profiling in order to get gene expression levels and isoform diversity at the single-cell level.