A salivary chitinase of Varroa destructor regulates mite-honey bee interactions

This dataset represents raw data and metadata underlying the findings (and the figures) reported in the manuscript "A salivary chitinase of Varroa destructor regulates mite-honey bee interactions". <strong>Fig 2A.</strong> Relative expression data of 3 selected candidates are presented as mean fold changes of 3-4 independent biological replicates. Each replicate consisted of a pool of 5-10 mites, from which the RNA was extracted from, salivary glands (SG), and from the rest of the whole body, deprived of salivary glands (Whole Body – SG). Values on Y-axis are reported in Log₁₀ scale. Error bars represent standard error of the mean. Statistically significant differences are denoted with an asterisk (P &lt; 0.005). <strong>Fig 4.</strong> <strong>Survival of <em>V. destructor</em> as affected by RNAi-mediated silencing of the gene encoding VdCHIsal. </strong>(A) Relative expression of <em>Vd-CHIsal</em> after soaking in a dsRNA solution. qRT-PCR data are presented as mean fold changes of 2-6 independent biological replicates. Each replicate consisted in a pool of 2-3 mites. Each time point was separately analyzed and the control sample was used as calibrator. Mean values within each time point were compared by Student’s t-test. Error bars represent the standard error of the mean. Mean values denoted with asterisks are significantly different (*p&lt;0.05; ** p&lt;0.01). (B) Kaplan-Meier survival curves of mites soaked in a solution of dsRNA targeting <em>Vd-CHIsal</em>. Host pupae were maintained throughout the feeding or replaced every 24 h. Error bars represent the standard error of the mean. <strong>Fig 5.</strong> <strong>Differentially expressed genes in honey bee pupae artificially infested with mites delivering saliva with the full repertoire of proteins or lacking Vd-CHIsal.</strong> (A) Differential expression of 13 honey bee genes, as affected by presence of Vd-CHIsal in the saliva (KD/WS). DESeq2 adjusted <em>P</em> was &lt; 0.05 and FDR was set at 5%. Log transformed mean FPKM values are reported on Y axis. Error bars represent SEM. Summary tables of differential expression analysis are presented in S2 and S3 Tables. (B) Relative expression of immune-related genes in honey bee pupae as affected by Vd-CHIsal expression in <em>Varroa destructor</em> infesting mites. Results of qRT-PCR are presented as mean fold changes relative to non-infested pupae used as calibrator. Values on Y axis are reported in Log₁₀ scale. Error bars represent the standard error of the mean. Mean values were compared by one-way ANOVA, followed by Tukey post-hoc test, and values statistically different denoted with different letters (P&lt;0.05). Detailed results of statistical analyses are presented in S4 Table. NP: non-parasitized controls; WS: pupae infested with mites soaked in saline solution; KD: pupae infested with mites soaked in <em>Vd-CHIsal</em> dsRNA solution.