Whole transcriptome sequencing of mouse cortical astrocytes (DIV 14).. Whole transcriptome sequencing of mouse cortical astrocytes (DIV 14).

To gain futher insight into the Npas3 regulatory network in astrocytes, RNA-seq was used to analyze the genome-wide changes resulting from the days in vitro (DIV) 14 astrocytes of P0 wild-type (WT) mice and littermate NPAS3-/- mice. Total RNA was extracted from DIV 14 astrocytes of P0 Npas3-/- and WT mice. Then, total RNA was quantified and quality controlled by Agilent Bioanalyzer 2100 system. After cDNA library construction, the library preparations were sequenced on an Illumina NovaSeq 6000 platform in Novogene. Overall design: Total mRNA profiles of DIV14 WT and Npas3-/- astrocytes (DIV14) were generated by deep sequencing using Illumina NovaSeq 6000.