The Molecular Dissection of the Oncogenic Role of ETS1 in the Mesenchymal Subtypes of Head and Neck Squamous Cell Carcinoma [ChIP-seq]

Mapping the genome-wide binding profile of ETS1 in a Mesenchymal HNSCC Cell Line using ChIP-Seq techniques The global binding loci of ETS1 in SCC25 cells, were mapped using ChIP-Sequencing analysis. We performed three replicate experiments of high quality, using two different antibodies, C20 (Santa Cruz Biotechnology) and 501A (Bethyl Laboratories), and consensus binding events were determined using IDR analysis. The ETS1 binding profiles in SCC25 were intersected with the global ETS1 Knockdown RNA-Seq analysis in order to identify the direct transcriptional targets of ETS1 in a Mesenchymal model of HNSCC.