Refolding of <i>Ta</i>AlDH from 10 g of <i>E. coli</i> cells produced in 1 L of fed-batch fermentation.
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1Protein concentration was determined with Bradford Assay/additionally after inclusion body washing and lyophilization by UV absorption spectroscopy. 2Enzyme activity was analyzed using NAD+ as electron acceptor during purification.
创建时间：
2015-12-02
