Fig2G_RACE_STOML2_R4_LG367_annotated.tif

Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), A/Tennessee/1-560/2009 (H1N1) (“H1N1pdm09” for 2009 pandemic H1N1) , or  A/Perth/16/2009 (H3N2) ("Perth"), or PA(∆X) mutants of these strains, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for STOML2 or YKT6, ~250-300 nucleotides (nt) downstream of the predicted cut sites. The PCR products were run on an agarose gel. The predicted size of DNA bands coming from cut sites identified by PyDegradome are indicated by the red dotted arrows.