A multi-omics approach incorporating single-cell RNASequencing reveals murine Leishmania major infection drives a proinflammatory signature alongside a signature of decreased ribosomal biogenesis.

RNA-seq analysis was performed in ear samples from naïve control and L. major infected mice. Using scRNASeq, we investigated the transcriptomic changes within individual cell types present in leishmanial lesion. Transcriptomic analysis through scRNASeq revealed 35 distinct cell populations which include both resident and recruited cell types following L. major infection. In addition, our scRNASeq analysis confirms a significant recruitment of various immune cell types such as macrophages, inflammtory monocytes, neutrophils, dendritic cells, CD4 and CD8 T cells in the infected ears that are seen at higher frequencies compared to naive controls. In overall, our scRNASeq data revealed that DEGs within each immune cell type driving a proinflammtory state during L. major infection, alongside a signature of decreased ribosomal biogenesis. Single-cell RNASeq of naïve control ears compared to ear samples from 4 week post infection with L. major promastigote parasites.