Rattus norvegicus Raw sequence reads. Rattus norvegicus

The underlying molecular mechanisms of ischemic stroke and the following reperfusion are still under investigation.To obtain a comprehensive, temporal-sensitive view of the cell-autonomous mechanisms involved in ischemic stroke and reperfusion, we applied the next-generation sequencing(NGS) technologyto characterize the temporal changes in gene expression profiles using primary cultured hippocampal neuronsunder an oxygen-glucose deprivation/reperfusion (OGD/R) condition. We first identified the differentially expressed genes (DEGs) between normal cultured neurons, neurons with OGD, and neurons with OGD followed byreperfusion for 6h, 12h, and 18h, respectively.We then performed bioinformatics analyses, including gene ontological (GO) and pathway analysis and co-expression network analysis to screen for novel keypathways and genes involved in the pathology of OGD/R. Finally, we validated the changes of selectedcore genes that might be responsible for the neuropathology of ischemia and reperfusion. Our research provided new insights into the molecular pathological mechanisms in ischemia stroke and potential targets for therapeutic intervention after ischemic stroke.