In vitro expansion of human sperm through nuclear transfer

We reported a kind of new haploid embryonic stem cell, human haploid androgenetic embryonic stem cell, which kept the sperm characteristic epigenetic modification patterns for imprinting genes. In this study, two human haploid androgenetic embryonic stem cell lines (ha-AGHESC) and two human haploid parthenogenetic embryonic stem cell lines (ha-PGHESC) with somatic control and diploid HESC control, were processed with RNA-sequencing (RNA-seq) and whole genome bisulfite sequencing (WGBS). We showed that the reconstructed semi-clone HESCs were similar to the diploid HESC in transcriptome and the methylome especially related to the known human imprinting genes. The raw data of WGBS and bulk RNA-seq are deposited at Genome Sequence Archive (GSA) of Human with accession number HRA004100. In this study, ha-AGHESC lines and ha-PGHESC lines each with two biological replications in early passages and elder passages were processed with RNA-seq and WGBS. One somatic sample, blood, and one HESC sample HUES42 were set as diploid controls. ICA1 and ICA2 were reconstructed diploid semi-clone HESC derived from semi-clone blastocysts that constructed by fusing ha-AGHESC with in vitro maturation (IVM) oocytes. Each sample had two technical replications. Please note that the processed data *1X_sort_CpG.bed files have been added for the GSM3737096-GSM3737117 samples on Jun 3, 2025.