ClHst1 associates with the rDNA repeats.

(A) Features of the three rDNA loci in C. lusitaniae are illustrated. The Pol I-transcribed 35S gene is processed to generate the 18S, 5.8S, and 25S rRNAs (arrows). The Pol III-transcribed 5S gene divides the non-transcribed spacer. All three loci occur near the ends of supercontigs, but only the right end of supercontig 3 has a telomere repeat (gray box), indicating the end of the chromosome. Vertical bars indicate the beginning (3L) or end (8R) of the supercontig sequence. (B) The association of ClHst1 across an rDNA repeat was examined by chromatin IP. Anti-myc antibody was used to immunoprecipitate proteins from ClHST1 (LRY2826) or ClHST1-MYC (LRY2858) yeast. The relative enrichment of each probe compared to a control locus, ClPRI2, is indicated.