Akt Activates NOS.

A) Western blot indicating that ISO increases Akt phosphorylation at S473 in a dose-dependent manner in isolated rabbit cells. B) ISO-dependent increase in p-Akt is blunted by Akt-Inhibitor X (top, right, * different from control, ** different from ISO, paired t-test, p<0.05). Cells were treated with ISO or ISO+Akt Inhibitor X. Akt Inhibitor X decreased the SR Ca leak and the activation of Akt (top left and bottom). C) Down-regulated Akt expression (plus the constitutively expressed Akt) increased the total Akt over the constitutive expression alone (top). Akt-dn decreased ISO-dependent SR Ca leak when data was selected to give the same average [Ca]SRT (bottom). D) NOS1 phosphorylation at Akt phosphorylation site S1416, representative immunoblot (left) and summary data (left). (* different from control, ** different from ISO, paired t-test, p<0.05).