RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cells. RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cells

We evaluated whether there are transcriptional differences between RNF31-proficient immune-resistant and RNF31-deficient immune-sensitive tumor cells. We stimulated sensitive (sgRNF31- or HOIPIN-8-treated) and resistant (sgCtl, vehicle) BLM tumor cells with TNF for 0 or 6 hours and performed RNA sequencing analysis. Tumor cells sensitized by RNF31 inhibition show a reduced canonical NF-kB and increased pro-death signature upon TNF challenge. Overall design: 1×10E6 BLM sgCtl or sgRNF31 BLM human melanoma cells were pretreated with vehicle (DMSO) or HOIPIN-8 (10 µM) for 16 h, followed by TNF stimulation (100 ng/ml) for 0 or 6 hours. Two replicates for each sample were performed.