TIPE drives a cancer stem-like phenotype by promoting glycolysis via PKM2/HIF-1α axis in melanoma

TIPE (TNFAIP8) has been identified as an oncogene and participates in tumor biology. However, how its role in the metabolism of tumor cells during melanoma development remains unclear. Here, we demonstrated that TIPE promoted glycolysis by interacting with pyruvate kinase M2 (PKM2) in melanoma. We found that TIPE induced PKM2 dimerization, thereby facilitating its translocation from the cytoplasm to the nucleus. TIPE-mediated PKM2 dimerization consequently promoted HIF-1α activation and glycolysis, which contributed to melanoma progression and increased its stemness features. Notably, TIPE specifically phosphorylated PKM2 at Ser 37 in an ERK-dependent manner. Consistently, the expression of TIPE was positively correlated with the levels of PKM2 Ser37 phosphorylation and cancer stem cell markers in melanoma tissues from clinical samples and tumor bearing mice. In summary, our findings indicate that the TIPE/PKM2/HIF-1α signaling pathway plays a pivotal role in promoting cancer stem cell ..., Transcriptomics 
Sample collection and preparation
RNA quantification and qualification
①     RNA degradation and contamination was monitored on 1% agarose gels.
②     RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA) .
③     RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).
Library preparation for Transcriptome sequencing
A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized u..., , # Data from: TIPE drives a cancer stem-like phenotype by promoting glycolysis via PKM2/HIF-1α axis in melanoma

[https://doi.org/10.5061/dryad.ghx3ffc05](https://doi.org/10.5061/dryad.ghx3ffc05)

## Description of the data and file structure

Transcriptomics Data

We conducted RNA sequencing analysis by overexpressing TIPE in G361 cells, which comprised three experimental groups and three control groups.

Metabolomics Data

We conducted a metabolomic analysis after interfering with TIPE in A375 cells, which consisted of six experimental groups and six control groups.

### Files and variables

#### **File: Metabolomics\_Data.xlsx**

**Description:** 

ID: The identification number of the metabolite in mass spectrometry detection
m/z: Mass-to-charge ratio

rt (s): Retention time of the metabolite on the chromatography, also known as the peak emergence time, with the unit being seconds

Name: The name of the metabolite

Adduct: Information on the adduct ions of the compound

Score: A scori...