five

Plasmodium falciparum (PfAMA1/PfMSP1)-specific heavy chain BCR sequence

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA695313
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For complete method see Hopp et al., JEM, 2021 (doi 10.1084/jem.20200901). Single probe-positive CD19+ CD10- IgM+ or IgG+ B cells were flow sorted from adult Malian PBMCs. The heavy chain of the BCR was amplified using reverse transcription and PCR followed by Sanger sequencing as previously described (Andrews et al., Immunity, 2019; Tiller et al., J. Immunol. Methods. 2008).
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2021-01-27
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