Modulation of miR-221, miR-222 and miR-1 expression upon myogenic differentiation.

RNAs extracted from QMb-ts and QMb induced to differentiate for 2 days, C2C12 mouse myoblasts and L6C5 rat myoblasts induced to differentiate for 2 (2d) and 4 (4d) days, and MSC induced to differentiate for 1 (1d) and 2 (2d) days were analyzed for the expression of miR-221, miR-222 and miR-1 by real-time RT-PCR. Fold decrease of miR-221 and miR-222 and fold increase of miR-1 expression in myotubes is shown for each cell type relative to levels detected in proliferating myoblasts. Standard error (s.e.) and statistical significance (p, n��3; for QMb and MSC, n = 3; for L6C5, n = 4; for QMb-ts, n = 6; for C2C12 n = 6) are shown. Not significant p values are underlined.