Gene expression changes in B-ALL upon oncogenic β-catenin activation [RNA-Seq]

We find that, in contrast to most cancer types, beta-catenin activation in preB-ALL cells supresses proliferation and leads to cell death. Proteomic analyses indicate that unlike in solid tumors beta-catenin interacts with Ikaros factors (IKZF1 and IKZF3) in pre-B cells. We performed RNA-seq in order to examine changes in expression upon beta-catenin activation in the presence and absence of Ikaros factors. RNA-seq was performed in pre-B cells from Ctnnb1Ex3fl/+ mice transformed with BCR-ABL1 and transfected with either non-targeting or IKZF1 and IKZF3 CRISPR/Cas9-RNP in order to study the effect of beta-catenin activation on gene expression in both IKZF1/3-WT and IKZF1/3-KO conditions.