Mus musculus Genome sequencing and assembly

We extracted total RNA from MH-S cells in si-NC group and si-Gm26917 group stimulated by LPS for 12 hours. The resulting cDNA library was sequenced using Illumina Novaseq6000 by Gene Denovo Biotechnology Co. To get high quality clean reads, reads were further filtered by fastp (version 0.18.0). The mapped reads of each sample were assembled by using StringTie v1.3.1 in a reference-based approach. For each transcription region, a FPKM value was calculated to quantify its expression abundance and variations, using RSEM software. RNAs differential expression analysis was performed by DESeq2 software between two different groups. The genes with the parameter of false discovery rate (FDR) below 0.05 and absolute fold changea bove or equal 2 were considered differentially expressed genes (DEGs). Differentially expressed genes were analyzed for GO or KEGG enrichment, and terms with a corrected P equal or lesser than 0.05 were considered significantly enriched in DEGs.