Data from: High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries

Microsatellites (or SSR: simple sequence repeat) are among the most
frequently used DNA markers in many areas of research. The use of
microsatellite markers is limited by the difficulties involved in their de
novo isolation from species for which no genomic resources are available.
We describe here a high-throughput method for isolating microsatellite
markers based on coupling multiplex microsatellite enrichment and
Next-Generation Sequencing on 454 GS-FLX Titanium platforms. The procedure
was calibrated on a model species (Apis mellifera) and validated on 13
other species from various taxonomic groups (animals, plants, fungi),
including taxa for which severe difficulties were previously encountered
using traditional methods. We obtained from 11,497 to 34,483 sequences
depending on the species and the number of detected microsatellite loci
ranged from 199 to 5,791. We thus demonstrated that this procedure can be
readily and successfully applied to a large variety of taxonomic groups,
at much lower cost than would have been possible with traditional
protocols. This method is expected to speed up the acquisition of
high-quality genetic markers for nonmodel organisms.