Additional file 1 of Addition of cariogenic pathogens to complex oral microflora drives significant changes in biofilm compositions and functionalities

Additional file 1: Figure S1. Impactsof both dietary sugar and pathogens on biofilm microbiota. A) Principlecoordinates analysis plot of Bray-Curtis dissimilarity between sucrose andglucose/fructose samples. X and Y axis show the percentage of total variancecaptured. B) Alpha-diversity of samples with different pathogens and differentcarbon sources. C) Difference in log2-transformed abundances of bacterialspecies as a result of adding S. mutants and/or C. albicans insucrose or glucose/fructose conditions. Bars indicate +/- standard error in thelinear mixed-effects model. Taxa shown are those that had FDR < 0.05 in thelinear mixed-effects model. Figure S2. Relative abundance of (A) S. mutansand (B) C. albicans in supernatant and biofilms. Figure S3. Alpha-diversitydifferences between biofilms and supernatants under all conditions. (p-value = 4.248396e-30for shannon and 4.168968e-25 for richness). Figure S4. Difference inlog2-transformed abundances of KEGG pathways in biofilm compared to the supernatantunder all conditions. Bars indicate +/- standard error in the linearmixed-effects model. Figure S5. Alpha-diversity differences in biofilms betweensucrose and glucose/fructose conditions. (p-value = 0.085 for Shannon and 0.067for richness). Figure S6. Relative abundance of fungal species inbiofilms. RPMM; Reads per Megabase per million mapped reads. Figure S7. Differencein log2-transformed abundances of fungal species in biofilm vs. supernatant.Bars indicate +/- standard error in the linear mixed-effects model. Abundance wascalculated as reads per megabase genome per million mapped reads. Figure S8.Confocal imaging of the biofilm morphology. The bacterial cells are labeledwith SYTO 9 (green), the C. albicans cells are labeled withConA-tetramethylrhodamine (blue), the human cells are labeled with DAPI (grey)and the EPS matrix is labeled with Alexa fluor 647 dextran (red). Scale bar:50µm. Table S1. Detection of S. mutans and C. albicansin saliva from healthy donors. Table S2. The changes of KEGGpathways in biofilm under different carbon sources and inoculums. Table S3. Thechanges of individual orthologs in biofilms under different carbon sources andinoculums.