Kinetics of osmotic stress regulate a cell fate switch of cell survival-Ramp10h -ID-101119-Jurkat cells exposed to hypertonicity

To understand the molecular changes of signaling and markers of cell state when cells are exposed to hypertonic stress, we measured time courses for the below markers: comb ramp10 combined comb ramp10 FSC_H comb ramp10 FSC_W comb ramp10 FSC_A comb ramp10 SSC_H comb ramp10 SSC_W comb ramp10 SSC_A combaba ramp10 SC_MKP1_FITC combaba ramp10 BD_pS6_A647 combaba ramp10 SC_NFAT5_A594 combabb ramp10 CS_aCaspase8_PE combabb ramp10 BD_aCaspase3_A647 combabc ramp10 BD_aCaspase3_PE combabc ramp10 BD_cPARP_A647 combabd ramp10 CS_pJNK_A647 combabd ramp10 CS_pMKK4_PE combabe ramp10 BD_IFNy_A700 combabe ramp10 BD_IL1b_PE combabf ramp10 BD_pH2AX_A555 combabf ramp10 BD_Ki67_A700 combabg ramp10 BD_pH2AX_A555 combabg ramp10 BD_FAS_PECy5 combabh ramp10 BD_pCREB_A647 combabh ramp10 BD_pmTOR_PE combabk ramp10 CS_p27kip1_PE combabk ramp10 CS_pHsp27_A647 combabi ramp10 CS_CHOP_A647 combabi ramp10 CS_LC3_PE combabj ramp10 CS_aCaspase9_PE combabj ramp10 BD_pNfkb_A647 combabp ramp10 CS_PP2AC_PE combabp ramp10 CS_pp38_A700 combabl ramp10 CS_p38_PE combabl ramp10 SC_Caspase12_A647 combabm ramp10 CS_pBad_PE combabm ramp10 BD_pMK2_A647 combabn ramp10 BD_FAS_PECy5 combabn ramp10 BD_FASL_PE combabo ramp10 BD_Bcl2_PE combabo ramp10 CS_BclXL_A647 combabq ramp10 SC_NOXA_A594 combabq ramp10 SC_ppp2ac_A680 combabr ramp10 RD_NLRP3_A700 combabr ramp10 CS_pATF2_PE combabs ramp10 SC_Bax_A647 combabs ramp10 CS_pIKKab_PE combabt ramp10 CS_pStat3_A647 combabt ramp10 CS_LC3b_PE combabu ramp10 SC_Bid_A647 combabu ramp10 BD_cPARP_PE combabv ramp10 CS_pp38_A594 combabv ramp10 SC_pASK_A680 combabw ramp10 BD_TNF_A700 combabw ramp10 CS_pcJun_PE combabx ramp10 BD_pBcl2_PE combabx ramp10 SC_HSP70_A680
Conclusion:
Conclusions are described in: https://www.biorxiv.org/content/10.1101/2020.07.10.197871v1
Notes:
timepoints 0,48,77,109,134,154,189,268,379,464,535,600 min after addition of 300 mosmol/l NaCl are barcoded by 12 barcodes using Pacific-Blue (dilution: high, mid, low) and Pacific-Orange ((dilution: high, midhigh, midlow, low) following established protocols for fluorescent cell barcoding for flow cytometry: timepoints are barcoded as follows: PO high + PB high=0, PO midhigh + PB high=48, PO midlow + PB high=77, PO low + PB high=109, PO high + PB mid=134, PO midhigh + PB mid=154, PO midlow + PB mid=189, PO low + PB mid=268, PO high + PB low=379, PO midhigh + PB low=464, PO midlow + PB low=535, PO low + PB low=600