The grass–microbiome-rumen axis in grazing sheep farming intervened by supplementary feeding

This study identifies key microbiome and epithelial cell subtypes involved in grass digestion and VFA metabolism in the rumen. By integrating multi-omic data, we reveal novel links between microbial activity, epithelial cell function, and grassland foraging, providing critical insights into mechanisms underlying grass prevalence and their implications for optimizing ruminant health and productivity. This research enhances our understanding of the grass-microbiome- rumen axis and its role in sustainable grazing systems. we selected six lambs using scRNAseg, tissues were collected from the ven_x0002_tral sac region of the rumen.