ETV4 mediates dosage-dependent prostate tumor initiation and cooperates with p53 loss to generate prostate cancer

Comparision of single cell transcriptome of prostate cells of T2,EYFP; T2,ETV4WT; T2,ETV4AAA and T2,ETV4AAA,p53L/L mice at 2 weeks and 4 months after TAM treatment Alive prostate cells of TMPRSS2-CreERT2;Rosa26-CAG-LSL-EYFP, TMPRSS2-CreERT2;Rosa26-CAG-LSL-ETV4WT, TMPRSS2-CreERT2;Rosa26-CAG-LSL-ETV4AAA and TMPRSS2-CreERT2;Rosa26-CAG-LSL-ETV4AAA;Trp53L/L mice at 2 weeks and 4 months after tamoxifen treatment were isolated by Fluorescence-activated cell sorting (FACS) according to DAPI and analyzed using scRNAseq.