Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform

The majority of cancer deaths are caused by solid tumors, where the four most prevalent cancers (breast, lung, colorectal and prostate) account for more than 60% of all cases (1). Tumor cell heterogeneity driven by variable cancer microenvironments, such as hypoxia, is a key determinant of therapeutic outcome. We developed a novel culture protocol, termed the Long-Term Hypoxia (LTHY) time course, to recapitulate the gradual development of severe hypoxia seen in vivo to mimic conditions observed in primary tumors. Cells subjected to LTHY underwent a non-canonical epithelial to mesenchymal transition (EMT) based on miRNA and mRNA signatures as well as displayed EMT-like morphological changes. Concomitant to this, we report production of a novel truncated isoform of WT1 transcription factor (tWt1), a non-canonical EMT driver, with expression driven by a yet undescribed intronic promoter through hypoxia-responsive elements (HREs). We further demonstrated that tWt1 initiates translation from..., , , # Data from: Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform

[https://doi.org/10.5061/dryad.cz8w9gjb4](https://doi.org/10.5061/dryad.cz8w9gjb4)

Contains RNASeq, ChIPSeq, Mass Spectrometry, and gPCR datasets used for the publication: Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform.

## Description of the data and file structure

Each file corresponds to a separate dataset. 

RNASeq:

    DEG_0.1vs5.complete.txt is the RNASeq file for long RNASeq analysis done during LTHY incubation (please refer to publication for methodology details). Sample names correspond to oxygen exposure levels. Table is DESeq2 processed output. Statistics were calculated comparing the beginning and the end of the experiment (5% oxygen vs 0.1% oxygen)

    miRs_0.1vs5.complete.txt is the RNAseq file for the same experiment as the DEG file. Sample naming conversion and file generation was the same as the D...