Phase-defined complete sequencing of the HLA genes by next-generation sequencing. Homo sapiens

We applied long-range PCR to amplify six HLA genes (HLA-A, -C, -B, DRB1, -DQB1, and PB1) followed by Nextera DNA Sample Preparation Kit and multiplex paired-end reads (2 x 250 bp) sequencing with the MiSeq sequencer. The Data Access Committee of the National Bioscience Database Center (NBDC) approved that this personal genetic data were made published according to NBDC data sharing guidelines (http://humandbs.biosciencedbc.jp/).