Chemically Induced Nuclear Pore Complex Protein Degradation via TRIM21

Despite the exciting progress of bifunctional degrader
molecules,
also known as proteolysis-targeting chimeras (PROTACs), the rapidly
expanding field is still significantly hampered by the lack of available
E3 ligase ligands. Our research bridges this gap by uncovering a series
of small-molecule ligands to the E3 ligase TRIM21 through DNA-Encoded
Library (DEL) technology. We confirmed their interaction with TRIM21
using crystallography and demonstrated their antiproliferative effects
across various cancer cell types. Furthermore, proteomic studies identified
that the mRNA Export Factor GLE1 and the Nuclear Pore Complex Protein
NUP155 were significantly downregulated on TRIM21 ligand treatment.
This degradation required TRIM21 and was ubiquitin-proteasome-dependent.
More specifically, NUP155 was the primary target for the TRIM21 ligands,
while GLE1 was considered a passenger target on initial degradation
of NUP155. Using immunofluorescence techniques, we further demonstrated
that the degradation of GLE1 and NUP155 proteins impaired the integrity
of the nuclear envelope, leading to cell death. Highlighted by this
research, a novel mode of action has been discovered for the TRIM21
E3 ligase ligand, acting as a monovalent degrader that triggers de
novo interaction with functional complex proteins and induces their
degradation.