Next Generation Sequencing analysis to compare expression patterns of myeloid progenitors in healthy donors and MDS patients

Transcriptional changes occurring in the CMPs and GMPs of untreated "CMP pattern" and "GMP pattern" MDS patients were compared with the transcriptional profile of CMPs and GMPs from healthy donors Lin-CD34+CD38+CD123+CD45RA- CMPs and Lin-CD34+CD38+CD123+CD45RA+ GMPs were FACS-purified from mononuclear cells previoulsy isolated by density gradient from fresh bone marrow aspirates and magnetically-enriched in CD34+ cells . Total RNA was purified using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific). Quality checks were performed in a 2100 Expert bioanalyzer (Agilent). cDNA libraries were prepared using the Ovation Single Cell RNA-Seq System (NuGEN Technologies). RNA-seq was performed with the HiSeq 4000 System (Illumina), generating 76-base pair reads. ***Submitter states that raw data will be submitted to dbGaP for controlled access***