S1PR1 Overexpression cells activates endogenous complement signaling and inflammasome to induce tumor metastasis in mice

Cancer metastasis remains one of the major causes of cancer deaths. The molecular mechanisms of pro-survival lipid signaling activation in inducing cancer cell migration and metastasis are largely unknown. Here, our RNA seq data showed that S1PR1 overexpression B16 melanoma cells injected in C57BL/6 mice via tail vein, activates endogenous complement signaling and inflammasome to induce tumor lung metastasis. C57BL/6 mice (Wildtype) and C3 knockout mice, were injected with S1PR1 overexpression B16 melanoma cell lines, via tail vein. Empty vector was injected in wildtype mice as control. After 21-30 days, mice were euthanized and lung extracted and stored in RNA stabilizer solution (ThermoFisher Scientific, cat# AM7022), until ready to use. Total RNA from mice lungs were isolated and sent for RNA sequencing.