RNA Sequencing analysis of primary HCASMC genetic expression following MK2i-NP treatment.

Primary HCASMCs that were untreated or underwent MK2i-NP treatment were compared based on genetic expression of ECM, inflammatory, and phenotype-specific genes. HCASMCs were cultured in 20% serum to induce phenotype switch. Treated cells were treated with MK2i-NPs for 2 hours at each passage. RNA was harvested at passage 5 when cells were ~80% confluent.