Curved crease origami at cellular scales enables hyper-extensibility of Lacrymaria olor

Eukaryotic cells undergo dramatic morphological changes during cell division, phagocytosis, and motility. Fundamental limits of cellular morphodynamics such as how fast or how much cellular shapes can change without harm to a living cell remain poorly understood. Here we describe hyper-extensibility in the single-celled protist Lacrymaria olor, a 40 μm cell which is capable of reversible and repeatable extensions (neck-like protrusions) up to 1500 μm in 20 seconds. We discover that a unique and intricate organization of cortical cytoskeleton and membrane enables these hyper-extensions that can be described as the first cellular scale curved crease origami. Furthermore, we show how these topological singularities including dcones and twisted domain walls provide a geometrical control mechanism for the deployment of membrane and microtubule sheets as they repeatably spool thousands of times from the cell body. We lastly build physical origami models to understand how these topological sin..., The contents of this dataset were used in the associated manuscript. A combination of microscopy (confocal fluorescence, scanning electron microscopy, transmission electron microscopy, high-speed DIC imaging) and physical models (origami) were all required to understand the ultrastructure of the membrane and cortical cytoskeleton in L. olor. From this data and its characterization and quantification, we were able to discover that the geometry and subcellular architecture within the cell gives rise to its unique hunting behavior: rapid, reversible hyper-extensions. , , # Curved crease origami and topological singularities enable hyperextensibility of *L. olor*

The contents of this dataset were used in the associated manuscript. A combination of microscopy (confocal fluorescence, scanning electron microscopy, transmission electron microscopy, high-speed DIC imaging) and physical models (origami) were all required to understand the ultrastructure of the membrane and cortical cytoskeleton in L. olor. From this data and its characterization and quantification, we were able to discover that the geometry and subcellular architecture within the cell give rise to its unique hunting behavior: rapid, reversible hyper-extensions.

**Description of the data and file structure**

This dataset contains several forms of data which were used in the manuscript. The file names are labeled by the headers: Figure_CollectionMethod_IdentifyingName. 

The Figure 2 headed file contains the alpha-tubulin-stained z-stack of a cell collected using a confocal microscope (Confoc...