Transcription profiling of C57BL/6NIA mouse hippocampus to survey age-related changes in mRNA levels

We carried out a global survey of age-related changes in mRNA levels in the C57BL/6NIA mouse hippocampus and found a difference in the hippocampal gene expression profile between 2-month-old young mice and 15-month-old middle-aged mice correlated with an age-related cognitive deficit in hippocampal-based explicit memory formation. Middle-aged mice displayed a mild but specific deficit in spatial memory in the Morris water maze. Experiment Overall Design: No technical replicates; 14 biological replicates for 15-month-old mice, 9 biological replicates for 2-month-old mice. Whole hippocampus.本研究对C57BL/6NIA小鼠海马组织中信使RNA（mRNA）水平的年龄相关变化进行了全局性分析，发现2月龄幼鼠与15月龄中年小鼠的海马基因表达谱存在差异，该差异与衰老相关的、依赖海马的外显记忆形成过程中的认知缺陷相关。在莫里斯水迷宫（Morris water maze）实验中，中年小鼠表现出轻度但具有特异性的空间记忆缺陷。实验整体设计：未设置技术重复；15月龄小鼠设置14个生物学重复，2月龄小鼠设置9个生物学重复，实验样本为完整海马组织。