genome skimming raw data for three species in Puya genus

Through genome skimming for Puya raimondii, P. macrura and P. hutchisonii, 70 pairs of cross-species SSR primers were selected and synthesized. 42 of 70 pairs of primers were successfully cross-species amplified. After preliminary screening, 12 SSR primer pairs produced clear bands and were used for the detection of potential polymorphisms in 84 Puya raimondii individuals collected from 4 populations in Peru. The number of alleles per locus ranged from one to 6, and the observed and expected levels of heterozygosity varied from 0.000 to 0.8929 and from 0.000 to 0.7662, respectively.