Pathogenic mechanisms implied by plasma exosomal miRNA profile of preeclampsia with severe features

Preeclampsia is a common complication of pregnancy that affects 4-5% of pregnant women around the world. At present, there is a lack of early identification of high-risk patients of preeclampsia in clinical practice, which restricts the development of disease prevention and treatment. Previous studies have indicated that plasma exosomal miRNAs in pregnant women could serve as biomarkers of preeclampsia, but few is focused on exosomal miRNAs from preeclampsia pregnancy with severe features(sPE). Therefore, we detected and compared the plasma exosomal miRNA profiles between normal pregancy and sPE to explore potential biomarkers and pathogenic mechanisms of sPE. Blood samples were collected from pregnant women in control group (normotensive pregnant women, n=3) and sPE group (patients with sPE, n=3). After exosome isolation and RNA extraction, exosomal miRNAs were profiled using next generation seqeuencing.